SDSTC LogoStanford Genome Technology Center 
Technology Development Group
 
Automated Sequencing System 

The massive amount of DNA sequencing necessary for the completion of the Human Genome Project and for the sequencing of other large genomes will require development of technology to increase sequencing throughput and to lower its cost. Our Technology Development Group, in collaboration with U.C. Berkeley and Affymetrix Inc., is developing a modular automated sequencing system with the goal of sequencing 1 Mb/day of assembled sequence at a cost of $0.01 per base. The development of this system is the main thrust of our technology development and is well under way. Three of the five main instruments involved in this system have been developed and are in use. These are the plaque picker, shaker, and M13 template preparation instrument. Together these instruments comprise the "Front-End" of the system and are in continuous use.  

The thermal cycler, sequencer, and plasmid purification instruments are under development. Automated editing, assembly, sample tracking and quality control software and hardware are also being developed. Automated finishing of sequence, including automated primer calling and synthesis, is planned for development once the shotgun path of the system is in place. 

 [Hydrodynamic DNA Shearer][Input Dish][Plate Server][Plaque Picker][Plate Server][14-Plate Cassette][Shaker][14-Plate Cassette][Plate Server][Template Preparation

Machine][Plate

Server][Thermocycler][Capillary Electrophoresis Sequencer][Oligonucleotide Synthesizer][Software

Development]
 
Staff | Instruments & Protocols | Automated Sequencing System | Functional Analysis | Software Development
Stanford Genome Technology Center
Technology Development Group
855 California Avenue | Palo Alto, CA 94304 | Phone: (650) 812-2007 | Fax: (650) 812-1975
wwwadmin@sequence.stanford.edu