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Technology Development Group
Functional Analysis

New sequencing technologies have both increased the rate of DNA sequence production and decreased the cost. Because of this, large amounts of new sequence are being generated, providing a wealth of data to be mined for information. Classical methods of single gene expression analysis cannot begin to keep up with this flood of new data. Genome-wide functional analysis is a burgeoning area of research focused on understanding global patterns of gene expression in an organism. One of the projects using the Microarrayer and Scanner involves the arraying of ESTs, or Expressed Sequence Tags, from the model plant Arabidopsis thaliana. In the pilot experiments, ~1500 separate DNA samples are spotted onto glass slides with the Microarrayer; these spots are arrayed in less than a square centimeter. The slides are then hybridized with fluorescently labeled cDNA probes made from RNA isolated from different plant tissues or from different stages of development. Differential gene expression of the entire array of samples can be assessed at once, providing a powerful analytical tool for understanding regulatory networks involved in the life of an organism.

For detailed information on the instruments used in Functional Analysis view these pages:
Arrayer
Arrayer
Scanner
Scanner
Oligonucleotide Synthesizer
Ologonucleotide
Synthesizer


Staff | Instruments & Protocols | Automated Sequencing System | Functional Analysis | Software Development

Stanford Genome Technology Center:
Technology Development Group
855 California Avenue | Palo Alto, CA 94304 | Phone: (650) 812-2007 | Fax: (650) 812-1975
wwwadmin@sequence.stanford.edu