Original kanB and kanC primers
The kanB primer is homologous to the non-coding strand and is located
250 bp downstream of the 5 '-end of thekanMX4 module.
The "A-kanB" primer pair is used to check the upstream recombination
kanB = 5 ' CTG CAG CGA GGA GCC GTA AT 3 '
The kanC primer is homologous to the coding strand and is located 587bp
upstream of the 3 ' end of the kanMX4 module.
kanC = 5 ' - TGA TTT TGA TGA CGA GCG TAA T - 3'
Alternate KanB and KanC primers
kanB1 is at position
252 on the KanMX map
kanB1 = 5'- TGT ACG GGC GAC AGT CAC AT - 3'
For the size of the A-KanB1 product, subtract90 from the size of A-KanB
given in the database
kanC3 is at position
1442 on the KanMX map
kanC3 = 5'- CCT CGA CAT CAT CTG CCC AGA T -3'
For the size of the KanC3-D product, subtract 450 from the size of
KanC-D given in the database.
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